浅谈工科院校《基因工程》课程中“载体”一章的教学设计

Study on the Teaching Design of Vector in Gene Engineering in Engineering Colleges

中国内地的基因工程教材都没有对质粒性质、大肠杆菌载体类型和酵母菌载体进行全面的讲述,文章综合6本书的内容设计了载体一章的教学。同时,扩展了穿梭载体的内涵,并纠正了碱裂解法提取大肠杆菌质粒的原理。

Characterization of Plasmid,vector type of E.coli and vector of yeast haven＇t been described comprehensively in the teaching material of Gene Engineering in China's Mainland,this article designed the teaching of Vector based on six books.The content of Shuttle Vector was extended and the principle of alkaline lysis method on extracting plasmid in E.coli was corrected.