不同来源小鼠胚胎培养在辅助生殖实验室质量控制中的应用

Application of the culture of mouse embryos from different sources to the quality control of the assisted reproductive laboratory

目的:利用小鼠胚胎体外培养技术对新建人类辅助生殖实验室进行质量控制,以保证培养体系的可靠性,并比较两种小鼠胚胎培养方法在实验室质量控制中的作用。方法:采用小鼠配子体外授精法和2-细胞胚胎收集法对胚胎进行培养,观察胚胎发育情况。结果:小鼠配子体外授精法的受精率为80.98%,受精卵的卵裂率、优质胚胎率及囊胚形成率分别为90.60%、68.15%、85.93%与收集的体内2-细胞卵胚胎的88.98%、70.33%、83.89%比较无显著性差异,P>0.05。结论:两种不同来源鼠胚培养方法均可作为实验室培养体系检测的评价指标。对于新建的胚胎培养实验室,小鼠配子体外授精法可对IVF的全过程进行检测,更能反映培养体系对胚胎的影响,同时还可熟练IVF的操作技能。

Objective： To use the in vitro culture technology of mouse embryos to conduct quality control of the newly-built human assisted reproductive laboratory so as to ensure the system＇s reliability,and compare the effect of two different ways of culturing mouse embryos on the laboratory＇s quality control.Methods： Embryos were cultured by in vitro fertilization（IVF） of mouse gamete and 2-cell embryonic collect method,and their embryo growth were observed.Results： The rate of fertilization for IVF of mouse gamete was 80.98%.The cleavage rate,high quality embryo rate and blastocyst rate of IVF of mouse gamete and 2-cell mouse embryonic collect method were 90.60%,68.15%,85.93% and 88.98%,70.33%,83.89% respectively,which were of no significant difference（P0.05）.Conclusion： The two different ways of culturing mouse embryos can be used as evaluation indicators in testing the culture system of the assisted reproductive laboratory.For the new-built embryo culture laboratory,the IVF of mouse gamete can monitor the whole process of IVF and also reflects the influence of the system on the culture of embryos and the mastery of the manipulative skill of IVF.