汉防己甲素肺靶向微球对大鼠低氧性肺动脉高压的急性效应

The acute effect of tetrandrine pulmonary targeting microspheres on hypoxic pulmonary hypertension in rats

目的 了解汉防己甲素 (Tet)肺靶向微球对低氧性肺动脉高压的治疗效应及其对肺循环的选择性作用。方法 采用喷雾干燥法制备Tet含量为 32 %的Tet肺靶向微球 ;雄性Wistar大鼠分为 3组 ,每组 10只 ,分对照组和Tet水溶剂治疗组、Tet肺靶向微球治疗组。经低氧处理 3周 ,分别给予Tet水溶剂和Tet肺靶向微球 ,观察用药前后大鼠肺动脉平均压 (mPAP)和体循环平均压 (mSBP)的变化。结果 低氧性肺动脉高压大鼠应用Tet肺靶向微球组mPAP的降低较Tet水溶剂治疗组更为明显 (P <0 .0 5 ) ,且mPAP在注药后 6 0min时仍明显低于注射前水平 ;大鼠mSBP无显著变化。结论Tet肺靶向微球对肺循环具有较好的选择性 ,可明显增强Tet降低mPAP的效应。

Objective To investigate the effect of tetrandrine (Tet) pulmonary targeting microspheres on hypoxic pulmonary hypertension and its selective role on pulmonary circulation. Methods Tet pulmonary targeting microspheres were synthesized by spraying desiccation, the content of tetrandrine in pulmonary targeting microspheres is 32%. Twenty Wistar rats were exposed to hypoxia for three weeks, the rats were given Tet pulmonary targeting microspheres and Tet aqueous solution respectively and another 10 rats were as normal control. The mean pulmonary arterial pressure (mPAP) was measured by a right cardiac catheterization, mean systemic blood pressure (mSBP) was measured by left femoral catheterization. Results At the end of hypoxic treatment, rats developed pulmonary hypertension. mPAP in the rats receiving Tet pulmonary targeting microspheres decreased more obviously than the rats receiving Tet aqueous solution ( P <0.05), and the effects maintained for 60 min after administration. However, the mSBP remained normal in rats receiving Tet pulmonary targeting microspheres. Conclusion Tet pulmonary targeting microspheres can improve the effect of Tet on the hypoxic pulmonary hypertension and Tet has the selective role on the pulmonary circulation.