小分子醇/盐二元双水相体系中盐酸土霉素的分配行为

Partition behaviors of oxytetracycline hydrochloride(OTC) in small molecule alcohol/salt binary aqueous two-phase system

在双水相体系研究的基础上,建立了乙醇与正丙醇和磷酸二氢钠形成的二元双水相体系萃取盐酸土霉素的新方法,考察了小分子醇的用量、盐种类和浓度、pH值、温度以及静置时间对盐酸土霉素分配行为的影响。结果表明,小分子醇/盐二元双水相体系可用于抗生素分配行为的研究,其中体系组成为乙醇和正丙醇,磷酸二氢钠浓度在48%,pH值在4～5,温度25℃,以及静置12 h左右,盐酸土霉素在该二元双水相体系中的分配系数达到21.95,萃取率达86.09%。

Based on the aqueous two-phase system,a new method of using ethanol and n-propanol with sodium dihydrogen phosphate binary aqueous two-phase system for extracting of oxytetracycline hydrochloride（OTC） was set up.The influence factors on partition behaviors of oxytetracycline hydrochloride（OTC） were studied,including the system composition,type and amount of salts,the pH value,the extraction temperature,and standing time after centrifuge.The results showed that the binary aqueous two-phase system can be used to study the distribution of antibiotics.When the system was ethanol and n-propanol,NaH2PO4 concentration of 48%,the pH value was 4~5,temperature was 25 ℃,and standing 12 h,the partition coefficient of oxytetracycline hydrochloride（OTC） was 21.95,and extraction rate was 86.09%.