洋葱胚性愈伤组织的诱导及增殖条件的研究

Induction and Proliferation Conditions of Onion(Allium cepa L.) Embryogenic Callus

[目的]探讨洋葱离体培养的最佳条件,为建立其高频再生体系及转基因研究奠定技术基础。[方法]以洋葱(Allium cepa L.)种子为外植体,对洋葱愈伤组织的诱导、胚性愈伤组织获得及增殖的培养条件进行了初步研究。[结果]不同培养基种类对愈伤组织的诱导有影响,BDS优于MS和B5培养基;当培养基中24,-D浓度为0.5 mg/L时种子出愈率最高;适当降低生长素浓度,添加分裂素BA能够促进胚性愈伤组织的发生,且无分化现象产生,胚性愈伤组织诱导最佳培养基为BDS+24,-D 0.25 mg/L+BA 2.0 mg/L,增殖最佳培养基为BDS+24,-D 0.25 mg/L+BA 1.0 mg/L。[结论]该试验建立的基因转化受体系统为洋葱基因转化奠定了良好基础。

[Objective] To explore the best conditions for the in vitro culture of onion, so as to lay technical foundation for its trangenic re- search and the establishment of high frequency regeneration system. [ Method ] With the onion seeds as explants, the induction and prolifera- tion conditions of onion （Allium cepa L. ） callus and embryogenic callus were preliminarily studied. [ Result ] The types of callus produced certain impact on the induction of callus, BDS was better than MS and B5 ; the callus rate was the highest at the concentrations of 0.5 mg/L 2,4-D ; the appropriate reduction of auxin concentration and addition of cytokinin BA could promote the induction of enbryogenic callus without differentiation, the best embryogenic callus induction medium was BDS ＋ 2,4-D 0.25 mg/L ＋ BA 2.0 mg/L and the best proliferation medium was BDS ＋ 2,4-D 0.25 mg/L ＋ BA 1.0 mg/L. [ Conclusion ] The transgenic receptor system established in this experiment lays good foun- dation for the genetic transformation of onion.