非繁殖季节中国美利奴羊血清E_2、P4、FSH、LH激素变化规律研究

The study on the variation laws for E_2,P4,FSH and LH in serum from Chinese Merino sheep during the non-breeding season

为了在非繁殖季节判断绵羊的发情状况,试验以新疆高海拔地区中国美利奴羊为研究对象,采用酶联免疫测定(ELISA)法测定绵羊血清中雌二醇(E2)、孕酮(P4)、促卵泡素(FSH)、促黄体生成素(LH)的含量,研究激素诱导发情羊、发情症状不明显羊、不发情羊体内激素分泌规律的差异。结果表明:激素诱导发情羊的E2含量比不发情羊高,差异显著(P<0.05);但是在P4、FSH、LH水平上,激素诱导发情羊要低于不发情羊,差异不显著(P>0.05)。发情羊与发情不明显羊在4种激素含量上差异不显著(P>0.05),说明公羊试情存在一定缺陷,而通过测定E2含量判定绵羊发情状态将成为未来研究的重点。

During non- breeding season, the Chinese Merino sheep at high altitude in Xinjiang were chosen as the experimental objects. The variation in content of E2 , P4 , FSH and LH were measured by enzyme -linked immunosorbent assay （ELISA）, the secretion differences of the four kinds of hormone among estrus sheep, anestrus sheep, and sheep with indistinct estrus symptoms were studied. The results showed that the estradiol （ E2 ） level of hormone - induced estrus sheep was higher than that of anestrus sheep, and the difference was significant （ P 〈 0.05 ）, but hormone - induced estrus sheep was lower than anestrus sheep in P4, FSH and LH levels, and the difference was not significant （ P 〉 0.05 ）. There were no significant differences between estrus sheep and sheep with indistinct estrus symptoms in E2 , P4, FSH and LH levels （ P 〉 0.05 ）. It indicated that there were some flaws in ram test. However, determining the estrus states in sheep will be the focus of future re- search through measuring E2 levels.