非洲猪瘟病毒检测试剂盒的研制

Development of African Swine Fever Virus Detection Kit

针对非洲猪瘟病毒(ASFV)P54基因建立荧光PCR检测方法,通过优化引物、TaqMan探针浓度及反应温度,研发了ASFV检测的荧光PCR检测试剂盒。经灵敏度的重复性试验及3种不同型号荧光PCR仪使用验证,所研发的试剂盒灵敏度为101copy/μL,适用于不同型号荧光PCR仪使用,可用于对非洲猪瘟病毒的快速检测。

A fluorescence polymerase chain reaction（PCR）assay was developed with ASFV p54 gene for the detection of African swine fever virus.And the ASFV fluorescent quantitative polymerase chain reaction（PCR）test kit was obtained through optimization of the primer and the fluorescence labeled TaqMan probe concentration and reaction temperature.Several snsitivity tests and verification with three different instrument models showed that,the sensitivity of kit was 101 copy/μL and suitable for different types of instrument.All these proved that the kit could be used for fast detection of ASFV.