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非生殖器部位脂溢性角化病组织中HPV L1蛋白及HPV16、18 DNA的检测及意义 被引量：2

Change and significance of HPV L1 protein and HPV 16,18 DNA in nongenital seborrheic keratosis
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摘要 目的观察非生殖器部位脂溢性角化病(SK)组织中HPV L1蛋白及HPV16、18 DNA的表达变化,并探讨其意义。方法分别采用免疫组织化学法、PCR法检测43例SK组织、15例正常皮肤组织中的HPV L1蛋白(HPV1、6、11、16、18、31)及HPV16、18 DNA。结果 SK组织中HPV L1蛋白阳性20例(46.51%),正常皮肤组织中HPV L1蛋白阳性4例(26.67%);两组阳性表达率比较,P>0.05。SK组织及正常皮肤组织中,均未检测到HPV16、18 DNA。结论非生殖器部位SK组织中并不存在HPV16、18 DNA,其发病与高危型HPV无关;但是非生殖器部位SK组织中有HPV1、6、11、31中某一型或者某几型L1蛋白的存在,属于非特异性感染。 Objective To observe the change of HPV L1 protein and HPV 16,18 DNA in nongenital seborrheic kera- tosis（SK） ,and explore its significance. Methods 43 SKs and 15 control specimens were detected for HPV L1 protein of HPV1,6,11,16,18 ,31 with immunofluoreseence technique, and HPV16,18 DNA with polymerase chain reaction （PCR）. Results HPV L1 protein of HPV1,6,11,16,18,31 expressed positive 20（46.51% ）SK and 4（26.67% ）normal tissue（P 〈 0.05 ）. There was no HPV16,18 DNA expression in 43 SK samples and 15 normal controls. Conclusion HPV16,18 DNA expression are not associated with nongenital SK. There are one or others subtypes HPV L1 protein of HPV1,6,11,31 in nongenital SK, belong to nonspecific infection.
作者 陈国仙 肖长义
机构地区 三峡大学医学院
出处 《山东医药》 CAS 2012年第18期20-22,共3页 Shandong Medical Journal
基金 国家自然科学基金资助项目(30770104) 湖北省自然科学基金资助项目(2011CDC002) 三峡大学硕士学位论文培优基金资助项目(2011PY034)
关键词 人乳头瘤病毒 免疫荧光化学法 聚合酶链反应 脂溢性角化病 human papilloma viruse immunofluoreseence technique polymerase chain reaction seborrheic keratosis
分类号 R758.7 [医药卫生—皮肤病学与性病学]
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参考文献14

  • 1Ko C J, Iftner T, Barr R J, et al. Changes of epidennodysplasia ver- ruciformis in benign skin lesions: the EV acanthoma[ J]. J Cutan Pathol, 2007,34( 1 ) :4448.
  • 2Tsambaos D, Monastirli A, Kapranos N, et al. Detection of human papillomavirus DNA in nongenital seborrhoeic keratoses [ J ]. Arch Dermatol Res, 1995,287(6) :612-615.
  • 3Lee ES, Whang MR, Kang WH. Absence of Human Papillomavir- us DNA in Nongenital Seborrhei Keratosis[J]. J Korean Med Sci, 2001,16(5) :619-622.
  • 4Akiyo G, Takuro K, Tamotsu K, et al. Detection and sequences of human papillomavirus DNA in nongenital seborrhoeic keratosis of immunopotent individuals [ J ]. J Dermatol Sci, 2003,31 ( 2 ) : 143- 149.
  • 5Li Y, Chen, Dong XP, et al. Detection of epidermodysplasia ver- ruciformis-associated human papillomavirus DNA in nongenital seb- orrhoeic keratosis[ J]. Br J Dermatol, 2004,151 (5) : 1060-1065.
  • 6Bai H, Cviko A, Granter S, et al. Immunophenotypic and Viral correlates of vulvar seborrheic keratosis [ J ]. Hum Pathol, 2005,34 (6) :559-564.
  • 7Perez LO, Barbisan G, Ottino A, et al. Human papillomavirus DNA and oncogene alterations in colorectal tumors [J]. Pathol On- col Res, 2010,16(3) :461-468.
  • 8Frank TS, Svoboda-Newman SM, Hsi ED. Comparison of methods for extracting DNA from formalin-fixed paraffin sections for noniso- topic PC R[ J ]. Diagn Mol Pathol, 1996,5 (3) :220-224.
  • 9黄幼生,宋伟伟,邓晓佳,罗志飞,解娜.四种微量石蜡组织DNA提取方法的比较[J].海南医学院学报,2010,16(9):1120-1123. 被引量：3
  • 10王丽江,甘润良,唐运莲,黄幼生,周建国.石蜡组织提取DNA4种方法的比较[J].诊断病理学杂志,2004,11(1):57-58. 被引量：7

二级参考文献11

  • 1Farrugia A,Keyser C,Ludes B. Efficiency evaluation of a DNA extraction and purification protocol on archival formalin-fixed and paraffin-embedded tissue[J]. Forensic Sci Int,2010, 194(1-3):e25-8.
  • 2Lehmann U, Kreipe H. Real-time PCR analysis of DNA and RNA extracted from formalin-fixed and paraffin-embedded biopsies[J]. Methods, 2001,25 (4): 409-418.
  • 3Goek SE, Hamilton SR, Vogelstein B. Purification of DNA from formaldehyde fixed and paraffin embedded human tissue[J]. Bioehem Biophys Res Commun, 1985,130 (13:118-126.
  • 4Cao W, Hashibe M, Rao JY, et al. Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cells[J]. Cancer Detect Prev, 200.3,27 (5):397-404.
  • 5Sato Y, Sugie R, Tsuchiya B, et al. Comparison of the DNA extraction methods for polymerase chain reaction amplification from formalinfixed and paraffin embedded tissues[J]. Diagn Mol Pathol,2001,10(4) :265-271.
  • 6Wu L, Patten N, Yamashiro CT, et al. Extraction and amplification of DNA from formalin-fixed, paraffin-embedded tissues[J]. Appl Immunohistochem Mol Morphol,2002,10(3):269-274.
  • 7Man YG, Moinfar F, Bratthauer GL, et al. An improved method for DNA extraction from paraffin sections[J]. Pathol Res Pract,2001,197(9):635-642.
  • 8Coombs NJ, Gough AC, Primrose JN. Optimisation of DNA and RNA extraction from archival formalin-fixed tissue[J]. Nucleic Acids Res,1999,27(16):12.
  • 9Sato Y,Sugie R, Tsuchiya B, et al. Comparison of the DNA extraction methods for polymerase chain reaction amplification from formalin-fixed and paraffin-embedded[J] tissues. Diagn Mol Pathol,2001,10(4):265-271.
  • 10王卡娜,李红芳,杨爱宏,许飞雪,杨永秀.石蜡包埋组织中DNA提取方法的比较[J].中国实验诊断学,2009,13(6):753-756. 被引量：3

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山东医药
2012年 第18期

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