32份水稻抗稻瘟病材料的SSR遗传多样性分析

Genetic Diversity of 32 Resistant Materials to Rice Blast Based on SSR Markers

为了对抗稻瘟病品种的选育及种质资源保护利用提供依据,利用24对SSR引物对筛选出的32份抗稻瘟病地方种质资源(品种)进行了遗传多样性分析。结果表明:24个位点共扩增出177个等位变异,平均每个位点7.375个,平均Nei’s基因多样性指数为0.7194,平均多态信息含量为0.6735,供试材料整体遗传变异较丰富,粳稻材料稍高于籼稻,但差异不显著;全部供试材料的平均遗传相似系数为0.2799,基因库保存的抗稻瘟病材料间的遗传差异大于近几年收集的地方抗病品种;基于Nei’s遗传相似系数的聚类分析,在0.34水平上将这些材料划分为籼稻和粳稻两个类群,亲缘关系的远近与其地理来源有一定的相关性。

Twenty-four pairs of SSR markers were used to analyze genetic diversity of 32 resistant materials to rice blast.The result showed that 177 alleles were detected with the average of 7.375.The average of Nei＇s genetic diversity index（He） and the polymorphism information content（PIC） were 0.719 4 and 0.673 5.This indicated these resistant materials had a relative high level of genetic diversity,and the genetic diversity of indica rice was slightly higher than that of Japonica one,but had no significant difference.The average genetic similarity coefficient was 0.279 9,and the genetic relationship among local resistance varieties were closer than that of materials come from gene bank.Clustering analysis showed when the similarity coefficient was 0.34 the material were divided into Indica and Japonica groups.The genetic relationship was some related to their geographical origin.