HPLC法测定痛风胶囊中青藤碱和大黄酸的含量

Determination of sinomenine and rhein in Tongfeng capsules by HPLC

目的建立痛风胶囊中青藤碱、大黄酸含量的测定方法。方法采用HPLC法,青藤碱含量测定的色谱柱为Agilent Eclipse Plus C18(5μm,4.6 mm×250 mm),流动相为乙腈-0.08%(体积分数)三乙胺(体积比21∶79),流速为1.0 mL.min-1,检测波长为262 nm;大黄酸含量测定的色谱柱为Kromasil C18(4.6mm×250 mm,5μm),流动相为甲醇-0.05%(体积分数)H3PO4(体积比85∶15),流速为1.0 mL.min-1,检测波长为254 nm。结果青藤碱在0.115 6～3.855μg范围内与峰面积呈良好的线性关系(r=0.999 5),平均回收率为99.48%,RSD为1.93%;大黄酸在0.083 2～0.832μg范围内与峰面积呈良好的线性关系(r=0.999 5),平均回收率为98.62%,RSD为1.13%。结论本方法专属性强、准确、重复性好,可用于痛风胶囊的质量控制。

Objective To develop a method for the determination of sinomenine and rhein in Tongfeng capsule.Methods The assay of sinomenine was performed on Agilent Eclipse Plus C18 column（5 μm,4.6 mm×250 mm） with a DAD（262 nm）.The mobile phase was acetonitrile-0.08% triethylamine（21：79） with a flow rate of 1.0 mL·min-1.The determination of rhein was on a Kromasil C18 column（5 μm,4.6 mm×250 mm） with a UVD（254 nm）.The mobile phase was methanol-0.05% phosphoric acid（85：15）.Results The linear range of sinomenine was 0.115 6-3.855 μg（r=0.999 5）,and the average recovery ratio was 99.48%（RSD=1.93%）;The linear range of rhein was 0.083 2-0.832 μg（r=0.999 5）,and the average recovery ratio was 98.62%（RSD=1.13%）. Conclusion The method is exclusive,accurate and reproducible,which may be applied in the quality control of Tongfeng capsule.