广宁红花油茶根尖压片制作技术

A Root Tip Squash Technique for Camellia semiserrata

广宁红花油茶在广东、广西等原生地种植容易获得高产,具有良好的遗传改良潜力。文章以广宁红花油茶为对象,探索了适用于荧光原位杂交技术中的染色体制片技术,并与传统的染色体压片制作技术进行比较,总结了一套节省时间、技术要求较低和效果较好的染色体根尖压片制作技术,可用于广宁红花油茶的细胞学研究,为开展分子染色体工程育种以及基因组分析奠定技术基础。

It is easier to acquire high yield of Camellia semiserrata if they are cultivated in their natural distribution area, such as Guangdong and Guangxi province. The yield character had a well potential of genetic improvement. The article summarizes a root tip squash technique for C. semiserrata. This technique could be applied on fluorescence in situ hybridization （FISH） study and it is easy to carry out, time saving and has better effect than traditional technique. It could also be applied on cytological studies, chromosome engineering and analysis of plant genome as a basic technique.