摘要
目的通过微透析-高效液相神经递质分析技术观察丘脑底核(subthalamic nucleus,STN)脑深部电刺激(deep brain stimulation,DBS)对偏侧帕金森(parkinson's disease,PD)模型猴苍白球内侧部(globus pallidusinternus,GPi)氨基酸类神经递质水平的影响。探讨PD的发病机制及DBS治疗PD的可能作用机制。方法成功制备的偏侧PD模型猴4只,微透析方法收集未注药侧GPi的细胞外液以及注药侧STN高频电刺激(highfrequncy stimulation,HFS)过程中以及刺激前后同侧GPi的细胞外液,应用高效液相-荧光法分析谷氨酸(gluta-mate,Glu)和γ-氨基丁酸(gamma-aminobutyric acid,GABA)的水平变化。结果 MPTP注药侧GPi细胞外液中Glu含量较未注药侧升高,而GABA含量却下降。高频电刺激偏侧PD猴模型的STN后,同侧的GPi细胞外液中Glu的含量明显上升,而GABA的含量无明显变化。结论无法用单一的神经元抑制机制解释STN-HFS的作用机制,STN-HFS的作用不只是局限于STN核团本身,对整个基底节环路都有影响。
Objective Extracellular recording and microdialysis-HPLC approach was used to observe the effects of high frequency stimulation of STN on amino acid neurotransmitters level of GPi in hemiparkinsonian model in rhesus monkey.The possible pathogenesis of PD and mechanisms of DBS was analyzed.Methods Four hemiparkinsonian monkeys were prepared with MPTP intracarotid injection.The coordinates of STN and GPi were verified by anatomy and electrophysioloigcal recording.On the MPTP injected side,extracelluar fluid of GPi was collected before,during and after ipsilateral STN-HFS,while on the contralateral side it was collected without STN-HFS.The level of Glu and GABA was measured with HPLC-fluorescence method.Results The level of Glu on MPTP-injected side was higher than that on non-MPTP-injected side,while the level of GABA on MPTP-injected side was lower.The level of Glu increased significantly after ipsilateral STN-HFS while that of GABA did not change.Conclusion The mechanism of STN-HFS could not be explained by the neuronal inhibitory effects.The effects of STN-HFS were not localized to the STN.On the contrary,other nuclei in the basal ganglia circuit might also be influenced.
出处
《立体定向和功能性神经外科杂志》
2012年第2期68-71,共4页
Chinese Journal of Stereotactic and Functional Neurosurgery
基金
北京市优秀人才培养资助项目(编号:20081D0300400069)
