杜仲饮片HPCE指纹图谱的研究

Study on HPCE Fingerprint of Eucommiae Cortex

目的:建立杜仲饮片HPCE指纹图谱分析方法,并对杜仲及其炮制品的指纹图谱进行比较。方法:采用高效毛细管电泳法进行色谱分离,以60 mmol/L硼砂-20 mmol/L磷酸二氢钠-10%甲醇(pH=10.0)为运行缓冲液,分离电压为20 kV,波长为210 nm,以松脂醇二葡萄糖苷为参照物,测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以10个共有峰为特征指纹信息的杜仲饮片HPCE指纹图谱;发现少数杜仲饮片的指纹图谱有一定差异,生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论:该方法准确可靠,重现性好,可作为杜仲饮片内在质量评价的依据。

Objective： To establish the analytical method for the fingerprint of Eucommiae Cortex by HPCE and compare the fingerprints of Eucommiae Cortex and its processed products.Methods： Based on the mode of high performance capillary electrophoresis,60 mmol/L sodium borate-20 mmol/L sodium dihydrogen phosphate-10% methanol（pH 10.0） was used as buffer solution.The separation voltage was 20 kV and the detection wavelength was set at 210 nm.Pinoresinol diglucoside was used as a reference standard,the chromatographic fingerprint were determined.The data were analyzed by fuzzy cluster and fingerprint similarity evaluation softwarewas used to compare the similarity of samples.Results： HPCE fingerprints with 10 common peaks of Eucommiae Cortex were established preliminarily.It was discovered that a small number of samples differed from others.Regarding to the fingerprints of Eucommiae Cortex and its processed products,there were obvious differences in the relative areas of common peaks.Conclusion： The method is reliable,accurate and can be used for quality control of Eucommiae Cortex.