牛蒡苷元制备方法的研究

Research on the Preparative Method of Arctigenin

目的:对在体外仿生提取牛蒡苷元的制备方法进行研究,优化筛选牛蒡苷元的酶水解条件、提取工艺等,得到最适工艺流程。方法:以酶浓度、酶解时间、底物浓度为考察因素,用Box-Behnken设计-响应面分析法对牛蒡苷元水解条件进行优化。结果:牛蒡苷元最佳制备条件:转化温度50℃,pH4.8,酶液浓度0.44 U/mL,反应时间46.81 min,底物浓度0.29 mg/mL,牛蒡苷元转化率可达到90.94%。结论:该研究为体外制备牛蒡苷元提供了一定的借鉴价值。

Objective： To research on the preparation of Arctigenin in vitro.Methods： Took enzyme concentration,time course and substrate concentration as investigation factors,used Box-Behnken design-response surface methodology to optimize the enzyme hydrolysis path of Arctigenin.Results： The best operational path for Arctigenin was as follows： the temperature was 50 ℃,pH was 4.8,enzyme concentration was 0.44 U/mL,time course was 46.81 min,substrate concentration was 0.29 mg/mL,the conversion rate was 90.94%.Conclusion： This research can be regarded as a reference in preparing Arctigenin in vitro.