舌癌组织IL-8-251单核苷酸多态性与复发

IL-8-251 single nucleotide polymorphism in the recurrence of squamouse cell carcinoma of tongue

目的:分析舌癌组织中IL-8-251位点的单核苷酸多态性与舌癌复发的关系,为预测舌癌的复发提供依据。方法:对142例舌癌患者的临床病理特点以及治疗手段等临床资料进行分析,用免疫组化方法检测IL-8在舌癌组织中的表达,高分辨率溶解法分析舌癌组织中IL-8-251位点的单核苷酸多态性,并分析IL-8-251多态性与舌癌复发和临床病理的关系。结果:142例舌癌患者中有52例复发,IL-8-251 3种基因型的频率在舌癌患者中分别为AA 14.8%(21例)、AT 47.2%(67例)、TT38%(54例),与对照组无明显差异;IL-8-251AA型的病例中12例复发,IL-8-251AT型的病例中23例复发,IL-8-251TT型的病例中17例复发,IL-8-251AA的舌癌患者复发率明显增高,且差异具有显著性;IL-8-251的多态性与性别、年龄、T分期、N分期、分化状态无关。结论:IL-8-251的多态性与舌癌复发密切相关,可作为预测舌癌复发的分子标记。

Objective： To observe the correlations between the local and cervical recurrence of squamous cell carcinoma of tongue and single nucleotide polymorphism of interleukin-8-251 in the tumor,and provide the evidence for the forecast of squamous cell carcinoma of tongue.Methods： Clinicopathological features and treatments of 142 cases with squamous cell carcinoma of tongue were reviewed and analyzed,and expression of interleukin-8 was detected by immunohistochemistry,single nucleotide polymorphism of interleukin-8-251 was identified by high-resolution melting.Results： 52 cases in this group had recurrence after operation,interleukin-8-251 was expressed in 99 cases,the gene type of IL-8-251 was AA 21 cases,AT 67 cases and TT 54 caes,12 recurrent cases in IL-8-251 AA,23 recurrent cases in IL-8-251 AT,17 cases recurrent cases in IL-8-251 TT.The recurrent rates of the group with IL-8-251 AA was high than the other group,no significance difference in gene type of IL-8-251 was observed with sex,age,T-category,N-category and differentiation.Conclusion： Local and cervical recurrence of squamous cell carcinoma of tongue has a significant relationship with IL-8-251 AA,and IL-8-251 AA could be a useful marker for forecasting the recurrence of squamous cell carcinoma of tongue.