不同灭菌法对续断中川续断皂苷Ⅵ含量的影响

THE STUDY ON THE EFFECT OF DIFFERENT STERILIZATION TO THE CONTENT OF DIPSACACEAE SAPONIN Ⅵ ON CHINESE TEASEL ROOT

目的考察在保证灭菌效率的前提下,不同灭菌法对续断中川续断皂苷Ⅵ含量的影响。方法分别采用不同方法灭菌后采用高效液相色谱法测定川续断皂苷Ⅵ灭菌前后的含量。结果水洗灭菌后川续断皂苷Ⅵ含量下降最多,为8.2%,而化学灭菌为4.6%、热灭菌为3.8%、辐射灭菌为2.1%。结论辐照灭菌最适合续断灭菌。

Objective Under guaranting the sterilization efficiency,to investigate the influnces on the content of the main active ingredient Chinese teasel root saponin Ⅵ by different sterilization.Methods After different sterilization,the indentify and variation on the contents of Dipsacaceae saponin VI was identified by HPLC.Results After washed sterilization on Dipsacaceae,the Dipsacaceae saponin Ⅵ decreased the most by 8.2%,while the chemical sterilization was 4.6%,heat sterilization was 3.8%,radiation sterilization was 2.1%.Conclusion Radiation sterilization was suitable for Chinese teasel root and had no obvious influence on the quality of the final product.