包涵体蛋白复性技术研究进展

Research progress in renaturation of inclusion body protein

本文对原核表达的包涵体蛋白的分离,洗涤,溶解及复性方法做了一个简单的总结。同时就国内外近几年的最新复性方法进行了概述。并分析了各种复性方法的利弊。

The isolation,washing,solubilization,and refolding of the inclusion bodies expressed from E.coli cells has been introduced in this review.At the same time,we evaluated some of the new refolding methods at home and abroad,especially on their.advantages and disadvantages.