纳米铂金颗粒Bioplatin的绿色合成、特征及其抗肿瘤作用(英文)

Green synthesis,characterization and anticancer potential of platinum nanoparticles Bioplatin

目的:研究纳米铂金颗粒的抗癌作用并分析其与小牛胸腺DNA及蜂蜜的相互作用。方法:使用绿色纳米技术合成纳米铂金颗粒Bioplatin并确定其物理特性如颗粒大小、电动电位及表面形态学。使用圆二色光谱分光光度法及傅里叶变换红外光谱技术以小牛胸腺DNA和蜂蜜作为靶点检测药物-DNA相互作用。使用噻唑蓝、荧光显微镜及DNA片段分析法检测其对外周血单核细胞及A375细胞的体外抗癌作用。结果:Bioplatin的纳米直径为137.5nm,表面电荷为-35.8mV。Bioplatin与DNA相互作用后对DNA的结构产生了明显的作用,使其发生改变,并形成了一种能够提高DNA稳定性的新的化合物。体外实验表明Bioplatin能够抑制细胞增殖,引起细胞核染色质凝固和DNA核小体断裂。结论:Bioplatin能够引起肿瘤细胞凋亡,因此具有一定的抗肿瘤作用。它能够与DNA相互作用,增加DNA的稳定性,从而抑制DNA的复制。

OBJECTIVE： In the present study, the anticancer potential of platinum nanoparticles Bioplatin is explored and the mode of interactions of Bioplatin with calf thymus DNA and honey was analyzed. METHODS： Bioplatin was synthesized with the help of green nanotechnology and characterized by particle size, zeta potential and surface morphology. The interaction of Bioplatin with DNA and honey was also checked with the help of circular dichroism spectroscopy and Fourier-transform infrared spectroscopy, respectively. The anticancer potential of Bioplatin was evaluated on peripheral blood mononuclear cells and A375 cells in vitro by analyzing results of MTT （3-（4,5）-dimethylthiahiazo-（-z-y1）-3,5-di-phenytetrazoliumromide）, fluorescence microscopic studies and DNA fragmentation assay. RESULTS： Bioplatin exhibited a small particle size of 137.5 nm and a surface charge of -35.8 mV. Bioplatin interacted with DNA and brought in effective changes in structure and conformation of DNA, and formed a new complex that increased its stability of DNA intercalated with the base pair of DNA. In vitro studies demonstrated that Bioplatin arrested cell proliferation, and induced chromatin condensation and internucleosomal DNA fragmentation. CONCLUSION： Bioplatin induces apoptosis in cancer cells and may have some beneficial effect against human carcinoma. It interacts with DNA, brings stabilization to DNA, and thus prevents the replication of DNA.