草药复方Bresol抵抗化合物48/80诱导的肥大细胞脱颗粒及组胺释放:促使肥大细胞稳定的一种非免疫机制(英文)

Polyherbal formulation Bresol protects the mast cells against compound 48/80-induced disruption and histamine release:a non-immunological mechanism of mast cell stabilization

目的:研究一种草药复方制剂Bresol对于肥大细胞脱颗粒以及组胺释放的保护作用。方法:使用大鼠腹膜内肥大细胞,在体外经化合物48/80诱导肥大细胞脱颗粒及组胺释放,评估Bresol稳定肥大细胞的作用。结果:显微镜下正常对照组涂片显示较多完整的肥大细胞,有极少量的脱颗粒肥大细胞和微量的组胺释放。阳性对照组中用化合物48/80培养的肥大细胞出现了显著的肥大细胞脱颗粒现象以及高浓度的组胺释放。而100mg/L浓度的Bresol明显抑制了化合物48/80诱导的肥大细胞脱颗粒。此外,Bresol可有效抑制化合物48/80诱导的组胺释放,且抑制效果与剂量有关。结论:Bresol能够在体外抑制化合物48/80诱导的肥大细胞脱颗粒和组胺释放。本研究的发现可解释Bresol对多种过敏疾病有效可能是通过一种非免疫机制。

OBJECTIVE： Present study was aimed to evaluate the protective effect of Bresol？, a polyherbal formulation, on mast cell degranulation and histamine release from mast cells. METHODS： Mast cell-stabilizing activity of Bresol？ was evaluated against compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells in ex vivo conditions. RESULTS： Microscopy of the control group smears showed more of intact mast cells, with very minimum number of degranulated mast cells and negligible amount of histamine release. In contrast, incubation of mast cells with compound 48/80 caused significant degranulation of the mast cells associated with release of high concentration of histamine in the positive control group. Furthermore, Bresol？ at 100 mg/L showed a significant inhibition of compound 48/80-induced mast cell degranulation. In addition, Bresol？ significantly and dose-dependently inhibited compound 48/80-induced histamine release. CONCLUSION： Bresol？ inhibits compound 48/80-induced mast cell degranulation and histamine release in ex vivo conditions. The present findings could be one of the non-immunological mechanism responsible for usefulness of Bresol？ in various allergic conditions.