HLRP蛋白在大鼠脑中的定位及LPS对其表达的影响

Distribution of HLRP in Rat Brain and Effects of LPS Lateral Ventricle Injection on HLRP Expression

目的:研究HLRP分子在大鼠脑中的细胞定位和表达特点,观察LPS刺激对动物脑HLRP表达的影响。方法:对原代培养的大鼠神经元、星形胶质细胞、小胶质细胞和大鼠脑组织冰冻切片分别进行免疫荧光染色,观察HLRP的细胞定位和表达特点;给大鼠侧脑室注射LPS,提脑组织蛋白,进行Western blot检测,半定量分析LPS刺激后,大鼠脑HLRP的表达变化。结果:①HLRP选择性表达于部分神经元的细胞核中,正常的星形胶质细胞和小胶质细胞不表达HLRP。②从嗅脑到脑干各节段,HLRP在大鼠脑组织中均匀分布,未发现HLRP阳性神经元聚集的现象。③侧脑室注射LPS 1天以后,HLRP表达明显升高(P<0.05)。结论:大鼠脑中正常表达HLRP,侧脑室注射LPS能刺激HLRP表达。

Objective： To investigate the location of HLRP on different cells in rat brain,and the effect of LPS lateral ventricle injection on HLRP expression.Methods： Neurons,astrocytes,and microglias were cultured seperately,and identified with anti-Tuj1,anti-GFAP,anti-OX42 IgG respectively.Then the three types of cells were co-labled with anti-HLRP IgG to investigate the location of HLRP.Discontinous frozen rat brain slices were gathered from olfactory brain to brainstem,then incubated with anti-HLRP IgG to observe the expression of HLRP.LPS solution was injected into rat lateral ventricle,and the expression of HLRP was analyzed with Westernblot method.Results： ① There was no HLRP expression in the neucleus of a certain subset of neurons,and astrocytes and microglias in normal conditions.② HLRP expression in rat brain showed no difference from olfactory brain to brainstem,and no HLRP positive neuron clusters were observed.③ After the injection LPS solution to rat lateral ventricle,HLRP expression increased obviously 1d later（P0.05）.Conclusion： HLRP expressed at normal rat neurons,and HLRP expression increased after injection LPS solution to rat lateral ventricel.