ESYT-2在致密核心囊泡分泌中起到的作用

ESYT-2 Regulates the Tethering Step of DCV Exocytosis in C.elegans

目的:研究秀丽隐杆线虫(简称线虫)中一种扩展的Synaptotagmin同源物ESYT-2在致密核心囊泡分泌过程中起到的作用。方法:以线虫为研究对象,运用线虫腔胞吸收ANF-GFP的基本原理来确定ESYT-2与分泌相关,之后又进一步使用全内反射荧光显微镜技术(TIRFM)来研究ESYT-2对致密核心囊泡的具体调控。结果:①ESYT-2功能缺失影响线虫神经细胞致密核心囊泡分泌。②ESYT-2影响致密核心囊泡分泌的栓系过程。结论:ESYT-2调控了致密核心囊泡的分泌过程。

Objective： To investigate the effect of ESYT-2 protein on DCV exocytosis in C.elegans.Methods： C.elegans was used as a model,neuropeptide release was assessed by measuring the fluorescence intensity of ANF-GFP in coelomocytes in vivo.Then the movements of single vesicles was tracked by using optical measurements（TIRFM）.Results： ① ESYT-2 loss of function animals exhibited a defect in DCV release.② ESYT-2 regulated the tethering step of DCV exocytosis in C.elegans.Conclusion： ESYT-2 affected DCV exocytosis in C.elegans.