父系HBeAg阳性流产胚胎绒毛中HBV-DNA的表达

Hepatitis B Virus Expression in Villi from Paternal HBeAg Positive Early Abortion Embryo

目的:探讨在父系HBeAg阳性的流产胚胎中,乙型肝炎病毒在绒毛中的表达。方法:募集仅父系感染乙型肝炎病毒组合,即母HBsAg(-)且父HBsAg(+)流产胚胎。按以下组合将入选对象分为4组:组1为父HBeAg(+)母HBsAb(+);组2为父HBeAg(+)母HBsAb(-);组3为父HBeAg(-)母HBsAb(+);组4为父HBeAg(-)母HBsAb(-),采用酶联免疫吸附实验(ELISA)对胎儿父、母亲血清进行乙肝抗原、抗体检测,并使用荧光定量PCR法对胚胎绒毛进行HBV DNA检测。结果:父系感染乙型肝炎病毒的142例胚胎中,仅在父系HBeAg阳性组别(1、2组)84例胚胎中发现3例绒毛HBV-DNA升高,阳性率为3.57%。其中父HBeAg(+)母HBsAb(-)组合中2例,父HBeAg(+)母HBsAb(+)组合中1例。父系HBeAg均阳性,母系HBsAb阳性与阴性组间子代绒毛HBV-DNA升高率差异无显著性(P>0.05)。结论:HBeAg阳性父亲可能更容易导致乙肝父婴垂直传播。

Objective： To investigate hepatitis B virus（HBV-DNA） expression in villi from paternal HBeAg positive early abortion embryo.Methods： Early abortion embryos,whose paternal serum HBsAg positive and maternal serum HBsAg negative,were collected.They were divided into G1 paternal HBeAg（＋）and maternal HBsAb（＋）,G2 paternal HBeAg（＋） and maternal HBsAb（-）,G3 paternal HBeAg（-） and maternal HBsAb（＋）,and G4 maternal HBsAb（-） and paternal HbeAg（-）.parents serum HBV antigen and antibody mea-surement was performed by ELISA,HBV-DNA expression in villi was measured by fluori-metric quantilative PCR.Results： Among 142 villi from paternal HBsAg positive early abortion embryo,3 of 84 villi from paternal HBeAg positive early abortion embryo were detect-ed for HBV-DNA expression.The positive rate was 3.57%.2 cases in G2 paternal HBeAg（＋） and maternal HBsAb（-）,and 1 case in G1 paternal HBeAg（＋） and maternal HBsAb（＋）.In groups whose paternal HBeAg were positive（G1 and G2）,there was no significant dif-ference for villi HBV-DNA expressing rate（P〉0.05）.Conclusion： Hepatitis B virus might more likely transmit vertically to early embryo villi in paternal HBeAg positive cases.