革皮氏海参皂苷单体抗血管新生的实验研究

The Experimental Study of Pearsonothuria graeffei saponins Inhibiting Angiogenesis Induced by Tumor Cells

研究革皮氏海参(Pearsonothuria graeffei)皂苷单体化合物echinoside A(EA)和ds-echinoside A(DSEA)对血管新生的抑制作用。采用MTT法和AO/EB荧光染色法研究EA和DSEA对人脐静脉血管内皮细胞Human UmbilicalVein Endothelial Cells(HUVEC)增殖、凋亡的影响;采用小管形成实验观察EA和DSEA对HUVEC分化形成小管能力的影响;细胞间粘附实验比较研究EA和DSEA对HUVEC细胞同肿瘤细胞之间粘附的影响;鸡胚绒毛尿囊膜(CAM)血管新生模型,观察EA和DSEA抑制CAM血管新生的情况。实验结果显示,EA和DSEA能够显著抑制HUVEC细胞的增殖(P<0.05,P<0.01),并诱导其凋亡;在体外能够显著抑制小管的形成能力,可显著抑制同肿瘤细胞间的粘附作用,在体内能够减少CAM新生血管的分支数目,而且DSEA的活性要强于EA。提示革皮式海参皂苷单体EA和DSEA都能够显著抑制肿瘤血管的新生,其活性与结构相关。

To investigate the effects of echinoside A（EA）and ds-echinoside A（DSEA）which were extracted from Pearsonothria graeffei on anti-angiogenesis,we MTT method and AO/EB staining were med to observe the influence of EA and DSEA on HUVEC cell proliferation and apoptosis.In addition,in vitro angiogenesis assay was applied to determine the effect of angiogenic ability of endothelial cells,also chick embryo chorioallantoic membrane（CAM） mode1 was used to check whether the neovascularization of CAM could be suppressed in vivo.The result indicated that EA and DSEA could decrease proliferation and induce apoptosis of HUVEC in dosage and time dependent manners.Moreover,both of them could inhibit the neovascularization in vitro and suppress angiogennesis of CAM in vivo.It is suggested that EA and DSEA can obviously inhibit the neovascularization and the inhibitory effect partially due to their structure.