与汉坦病毒β3整合素受体结合的高亲和性多肽的筛选

Screening of the high affinity peptides binding to β3 integrin receptor of hantavirus from phage display random peptide library of hantavirus receptor

目的从噬菌体七肽展示库中筛选与β3整合素结合的多肽序列,并进行鉴定。方法以β3整合素为靶分子对噬菌体七肽展示库进行8轮亲和筛选,获得与β3整合素结合的噬菌体克隆,挑选结合力强的克隆进行DNA测序,推导出呈现的多肽序列,应用生物信息学软件进行多肽序列分析和同源性分析。结果经8轮亲和筛选从噬菌体展示七肽库中挑选80个克隆进行DNA序列测序及生物信息学分析,发现TGVKGPG、LPLTPLP、KLTSSPT、SPVGPLP和DHRNHLV肽段有较高的重复机率,与汉坦病毒囊膜糖蛋白氨基酸序列有较高的同源性。结论通过对噬菌体展示肽库的淘筛,获得与β3整合素结合的高亲和性多肽,为进一步设计和获取高活性的抗汉坦病毒短肽提供参考资料。

Objective To screen and identify peptides with high affinity to β3 integrin receptor of hantavirus from phage display random peptide library. Methods β3 integrin receptor was taken as a ligand, phage clones that bond to β3 integrin receptor were screened from phage display random 7-mer peptide library through 8 rounds of biopanning. The phage clones containing high affinity peptides to β3 integrin receptor were selected and amplified. The DNAs were extracted for sequencing. The coding peptide sequences were deduced from the DNA sequences and analyzed for bioinformatics, including homology analysis through blasting. Results Five peptides （TGVKGPG,LPLTPLP,KLTSSPT,SPVGPLP and DHRNHLV） revealed high repetition rate. The comparison of the amino acid sequences with hantanvirus glycoprotein showed high homology. Conclusions Peptides with high affinity to β3 integrin were obtained from phage display random peptide library, which provide new data for further experiments to get the highly active anti-hantaviral peptides.