HIV/AIDS患者机体氧化还原态研究

Reasearch on the redox status in patients with HIV/AIDS

目的探讨HIV/AIDS患者机体的氧化还原态特点。方法应用流式细胞术检测80例抗-HIV阳性患者血中CD4细胞水平,根据CD4水平将80例患者分为两组:AIDS组50例、HIV感染组30例。选取健康体检者30例为对照组。取患者静脉血,检测血浆还原型谷胱甘肽(GSH)与氧化型谷胱甘肽(GSSG),还原型辅酶Ⅱ(NADPH)与氧化型辅酶Ⅱ(NADP+),计算GSH/GSSG和NADPH/NADP+的氧化还原电位,评价HIV/AIDS患者机体氧化还原态。结果 HIV/AIDS患者血浆氧化还原态向氧化方向偏移。结论氧化还原态失衡、氧化还原态向氧化方向偏移与AIDS发生、发展密切相关。

Obsjective To investigate the redox status in HIV/AIDS patients. Methods The levels of CD4 were detected by flow cytometry in 80 patients with HIV antibody-positive. The patients were divided into two groups： AIDS group （n= 50） and HIV infection group （n= 30）.Healthy people were taken as control group （n= 30）. Plasma glutathione （reduced form GSH and oxidized form GSSG） were measured by fluorescence spectrophotometry, reduced form NADPH and oxidized form NADP＋ were measured by UV spectrophotometry. Redox status was evaluated by GSH/GSSG and NADPH/NADP＋. The GSH/GSSG and NADPH/NADP＋ redox potential were calculated according to Nernst equation. Results The GSH/GSSG and NADPH/NADP＋ redox status in plasma of patients with HIV/AIDS deviated to pro-oxidative direction. Conclusions The imbanlance and deviation to pro-oxidative direction of redox status closely related with the occurrence and development of AIDS.