水稻抗条纹叶枯病分子标记在不同品种中的扩增比较

Comparison of amplified molecular markers in different rice varieties with stripe disease resistance

以感水稻条纹叶枯病品种"日本晴"、高抗品种"kasalath"以及14个目前在我国长三角地区水稻生产中被认为在抵抗条纹叶枯病方面具有较好效果的水稻品种为材料,分析比较了14对分子标记引物扩增16个水稻品种基因组获得的DNA产物.结果显示:在14对分子标记引物中,有8对在扩增感病品种"日本晴"和高抗品种"Kasalath"水稻基因组时可获得多态性条带.因此推断这8个标记可在以这两个品种为亲本的抗条纹叶枯病选育中使用;并且,本研究结果还将为利用本试验收集的14个水稻品种为亲本的分子标记辅助育种提供重要的背景信息.

The PCR products,which were amplified from the genomic DNA of the susceptible ″Nipponbare″ rice and stripe disease resistance rice ″Kasalat″h and other fourteen rice varieties considered to have good resistance to stripe disease of rice varieties planted in the Yangtze River Delta region,were analyzed using 14 pairs of molecular marker primers of stripe disease resistanc.The results showed that in 14 pairs of primers of molecular markers,there are 8 pairs of polymorphic bands in both amplified susceptible cultivar ″Nipponbare″ and high-resistant variety Kasalath rice genome.It is deduced that these 8 marks of stripe disease resistance in the two species could be used in rice cultivar selection when these two varieties were as parents;and the results also provided an important background information for breeding new varieties by molecular marker-assisted selection when the 14 rice varieties would be used as hybrid parents.