p53蛋白、ki-67抗原和表皮生长因子受体在胃癌组织的表达及意义

Clinical significance of p53 protein,ki-67 antigen and epidermal growth factor receptor expression in gastric carcinoma tissue

目的探讨胃癌组织中p53蛋白、ki-67抗原和表皮生长因子受体(EGFR)表达与TNM分期的关系。方法回顾性分析胃癌手术标本66例,采用免疫组织化学MaxVisionTM2/HRP法检测66例胃癌组织中p53、ki-67和EGFR的表达,分析其临床病理特征及与预后的关系。结果各分子标记物在胃癌组织中的阳性表达率为:p53蛋白53.03%(35/66),ki-67抗原62.12%(41/66),EGFR 42.42%(28/66)。P53蛋白表达与胃癌病灶直径、淋巴结转移密切相关(P<0.05),ki-67抗原阳性表达与胃癌分化密切相关(P<0.05),EGFR阳性表达与胃癌浸润深度、淋巴结转移密切相关(P<0.05)。p53、ki-67、EGFR三者联合检测与胃癌TNM分期密切相关(P<0.05)。结论 p53、ki-67、EGFR三者联合检测优于单独检测,与胃癌TNM分期相关。

Objective To evaluate the expression of p53 protein,Ki-67 antigen and Epidermal Growth Factor Receptor（EGFR） in gastric cancer（GC） and the correlation between clinicopathological features and prognosis.Methods Tissue samples from 66 patients treated by gastric resection for GC between 2006 and 2010 in our hospital were used.Formalin-fixed paraffin-embedded tumor tissue were retrospectively studied by immunohistochemistry,using monoclonal antibodies to p53,Ki-67 and EGFR.The results were correlated with clinicopathological features and prognosis.Results Positive expression rates of p53,Ki-67 and EGFR were 53.03%,62.12%,42.42% respectively.P53 expression were correlated with tumor size and regional lymph node metastasis（P0.05）.Ki-67 expression were correlated with histological grade（P0.05）.EGFR expression were correlated with infiltration depth and regional lymph node metastasis（P0.05）.Combined analysis of p53 protein,Ki-67 antigen and EGFR were correlated with pTNM stage（P0.05）.Conclusion Combined analysis of p53,Ki-67 and EGFR has more advantage than that of anyone alone for pTNM stage in gastric cancer.