温肾补阳法对变应性鼻炎患者转录因子T-bet/GATA-3表达的影响

Influence of Wenshen Buyang method against transcription factor T-bet/GATA-3 of patients with allergic rhinitis

目的:研究变应性鼻炎患者转录因子T-bet/GATA-3的表达及温肾补阳法对其作用。方法:选择符合诊断标准(中医肾阳虚型;西医属常年性变应性鼻炎)的患者91例,治疗组41例,对照组50例,另选择正常健康的受试者20例作为空白对照组。治疗组采用金匱肾气丸合苍耳子散为主方加减治疗;对照组采用氯雷他定治疗,疗程4周。本实验采用酶联免疫吸附测定(ELISA)法检测变应性鼻炎患者外周血淋巴细胞培养上清液中白细胞介素4(IL-4)和r干扰素(INF-r)浓度,用逆转录-聚合酶链反应(RT-PCR)和流式细胞术(FCM)测定淋巴细胞中T-bet、GATA-3mRNA表达水平。结果:温肾补阳组患者治疗后同治疗前相比,INF-r、T-bet水平明显提高(P<0.05),IL-4、GATA-3水平明显下降(P<0.05),同正常对照组相比无明显差别(P>0.05)。而氯雷他定治疗前后无明显改善。结论:温肾补阳法可通过调节T-bet/GATA-3表达,纠正失衡的Th1/Th2细胞因子网络,从而对变应性鼻炎产生治疗作用。

Objective： To study influence of Wenshen Buyang method against transcription factor T-bet/GATA-3 of patients with allergic rhinitis. Methods： 91 cases were divided into the treated group （41） and the control group （50）, normal and healthy subjects 20 cases were selected as a blank control group. The treated group was given Jinkui Shenqi Wan plusXanthium powder; the control group was given loratadine, 4 weeks for a course. Results： Wenshen Buyang group, INF-r, the level of T-bet was significantly increased （P0.05） compared to pre-treatment, compared with the normal control group, no significant difference （P0.05）. Conclusion： Wenshen Buyang method was effective in treating allergic rhinitis.