僵蚕提取物对棉铃虫的胃毒作用和碱性磷酸酶活性的影响

Toxicity and Alkaline Phosphatase Activity Inhibition of the Extracts from Bombyx batryticatus Against Helicoverpa armigera

僵蚕提取物对棉铃虫有胃毒作用,对饲毒后不同时间棉铃虫3龄幼虫体内碱性磷酸酶的活性进行了研究。结果表明,在3龄棉铃虫人工饲料中添加僵蚕提取物浓度为3mg·g-1时,饲毒第3天试虫的校正死亡率为66.73%;5mg·g-1的剂量导致试虫死亡率可以高达83.33%,表明僵蚕提取物对棉铃虫具有很强的胃毒作用。试虫碱性磷酸酶活性分析表明,1～5mg·g-1浓度处理组的试虫碱性磷酸酶活性与对照组相比均显著下降,并有明显的剂量相关性。推测僵蚕对棉铃虫幼虫的毒杀作用机制之一与其对碱性磷酸酶活性的抑制有关。

This paper reported the study results on feeding toxicity and inhibition effects on alkaline phosphatase（ALP） of Helicoverpa armigera treated with the extracts of Bombyx batryticatus.The corrected mortality of H.armigera treated with the extracts of B.batryticatus at 3 mg·g^-1 concentration in insect diet was 66.7% after feeding on the toxic diet three days,while the corrected mortality was up to 83% to the larvae treated with a concentration of 5 mg·g^-1,indicating that the B.batryticatus extracts was high toxic to the third instar larvae of H.armigera.The enzyme assay showed that the ALP activities decreased significantly while treated with the B.batryticatus extracts concentration ranged from 1 mg·g^-1 to 5 mg·g^-1,and the correlationship was responsible to the dosage of B.batryticatus extracts.Thus,the inhibition on ALP activity is a possible mechanism for the pesticidal activity of B.batryticatus extracts.