中华鳖5个群体遗传多样性的微卫星分析

Genetic Diversity in Five Populations of Trionyx sinensis Revealed by Microsatellite Markers

利用微卫星分子标记技术对洞庭(DT)、黄河(HH)、黄沙(HS)、日本(RB)以及洞庭(DT)与黄河(HH)的杂交子代(DT♀×HH♂)绿卡(LK)5个中华鳖群体的150个个体进行遗传多样性分析。11对微卫星引物扩增出的等位基因数为3～6个,平均等位基因数为4.1818。5个种群相比,绿卡(LK)的平均有效等位基因数、平均期望杂合度、平均观测杂合度和平均多态信息含量最高,分别是2.3969、0.5274、0.5545和0.4660。对种群间的遗传分化分析表明,黄河(HH)和洞庭(DT)之间的遗传分化最小,为0.0233,而洞庭(DT)和日本(RB)之间的遗传分化最大,为0.0969。基于Nei's遗传距离构建的UPGMA系统进化树显示黄河(HH)和洞庭(DT)及其子代绿卡(LK)亲缘关系较近,而与黄沙(HS)和日本(RB)的亲缘关系较远,最远的为日本(RB)群体。

In this paper,microsatellite markers were used to analyze the genetic diversity of 150 individuals from five Trionyx sinensis populations：Dongting（DT）,Yellow River（HH）,Huangsha（HS）,Japan（RB）,and Luka（LK） which is the filial generation of Dongting（DT） and Yellow River（HH）,The number of alleles amplified from 11 pairs of microsatellite primers is 3 to 6,and the mean allele number is 4.181 8.The mean effective number of alleles（Ne）,mean expected heterozygosity（HE）,mean observed heterozygosity（HO） and mean polymorphism information content in LK population are all the highest among these five populations,and there are 2.396 9,0.554 5,0.527 4,and 0.466 0 respectively.FST index between every two populations at all loci indicates that the genetic differentiation between HH and DT is the smallest,0.023 3;while the genetic differentiation between DT and RB is the largest,0.096 9.The UPGMA dendrogram based on Nei＇s genetic distance shows that DT,HH and LK are grouped in one cluster,which is then clustered with HS.The RB population is grouped in a separate cluster.