MMSET蛋白在肺癌组织芯片中的表达

Expression of MMSET protein in lung cancer tissue microarray

目的探讨组蛋白甲基转移酶(MMSET)在肺癌组织中的表达及临床意义。方法应用组织芯片技术结合免疫组化法,检测125例肺癌组织和11例肺良性病变组织MMSET的表达。结果 MMSET在肺良性病变组织中表达阴性;在肺癌组织中的表达阳性率为46.4%(58/125),其中在小细胞肺癌、肺鳞癌、肺腺癌中分别为85.7%、9.7%、3.2%;,在小细胞肺癌组织中的阳性率显著高于肺鳞癌及肺腺癌(P<0.05);此外,MMSET在小细胞肺癌(SCLC)与非小细胞肺癌(NSCLC)间的表达差别有统计学意义(P<0.05),MMSET在低+未分化组肺癌的表达高于高+中分化组(P<0.05),在Ⅲ+Ⅳ期肺癌的表达高于Ⅰ+Ⅱ期(P<0.05),有淋巴结转移组表达高于无淋巴结转移组(P<0.05)。结论 MMSET基因可能是肺癌的一种癌基因,MMSET的表达与肺癌组织学类型、分化程度、临床分期、淋巴结转移有关,也与肺癌的发生、发展有关,有望成为一种新的有助于肺癌诊断和治疗的标志物。

Objective To investigate the expression of MMSET protein in tissues of lung cancer and the clinical significance.Methods The expression of MMSET in 125 cases of lung cancer tissues and 11 cases of lung benign lesion tissues was detected by tissue microarray technique combined with immunohistochemistry.Results The expression of MMSET in the lung benign lesion tissues was negative.The positive rate of MMSET expression in the lung cancer tissues was 46.4%（58/125）.The positive rates in small-cell lung cancer（SCLC）,lung squamous-cell carcinoma and adenocarcinoma of lung were 85.7%,9.7% and 3.2%,respectively.The positive rate in SCLC was significantly higher than those in lung squamous-cell carcinoma and adenocarcinoma of lung（P0.05）.In addition,there were significant differences in the expression of MMSET between SCLC and NSCLC（P0.05）.The expression of MMSET in the group of low-undifferentiation lung cancer was higher than that in the group of high-middle differentiation（P0.05）.That in the Ⅲ＋Ⅳ stage of lung cancer was higher than that in the Ⅰ＋Ⅱstage（P0.05）.And that in the group with lymph node metastasis was higher than that in the group without lymph node metastasis（P0.05）.Conclusion The MMSET gene may be an oncogene of lung cancer.The expression of MMSET is closely related to histology type,differentiation degree,clinical stage,and lymph node metastasis of lung cancer.It is also related to the occurrence and growth of lung cancer,which may be a new marker for the diagnosis and treatment of lung cancer.