322份血培养阳性病原菌在需氧、厌氧瓶的检出率比较

Comparison of detection rates of 322 blood culture positive pathogens in aerobic and anaerobic bottles

目的分析2010年7月-2011年7月血培养阳性的病原菌在需氧、厌氧瓶检出率。方法血培养及鉴定采用美国BD9050全自动血培养仪和法国生物梅里埃公司ATBExpression自动细菌鉴定仪。结果在2700份血培养中阳性322份,阳性率为11.9%,其中革兰阳性球菌占24.5%、革兰阳性杆菌占0.6%、革兰阴性球菌占0.3%、革兰阴性杆菌占65.8%、厌氧菌占2.0%、真菌占6.8%;其中仅需氧血培养阳性130份,占40.4%,仅厌氧血培养阳性60份,占18.6%,需氧与厌氧血培养均阳性132份,占41.0%。结论同一患者同时进行需氧瓶及厌氧瓶培养,即能充分体现自动血培养仪快速性,又能提高血培养阳性率。

OBJECTIVE To analyze the detection rates of blood culture-positive pathogens in the aerobic bottle and anaerobic bottle from Jul 2010 to Jul 2011.METHODS The culture and identification for the blood were performed with the United States BD 9050 automated blood culture system and French bio-Merieux ATBExpression automatic bacterial identification system.RESULTS Of 2700 blood culture specimens,322 blood cultures were positive,the positive rate was 11.9%,including gram-positive cocci（24.5%）,gram-positive bacilli（0.6%）,gram-negative cocci（0.3%）,gram-negative bacilli（65.8%）,anaerobic（2.0%）,and fungi（6.8%）;there were only 130（40.4%） positive blood cultures in aerobic bottle and 60（18.6%） positive blood cultures in anaerobic bottle;there were 132（41.0%） positive cultures in aerobic and anaerobic blood cultures.CONCLUSION Simultaneous culture with aerobic and anaerobic bottles for the same patient not only can fully reflect the rapidity of automatic blood culture system of rapid but also improve the positive rate of blood culture.