高效液相色谱法同时测定环境水中5种邻苯二甲酸酯

Simultaneous Determination of Five Phthalate Acid Esters in Environmental Water by HPLC

建立了萃取-高效液相色谱法对环境水样中的5种邻苯二甲酸酯类化合物(PAEs)[邻苯二甲酸二甲酯(DMP),邻苯二甲酸二乙酯(DEP),邻苯二甲酸二丁酯(DBP),邻苯二甲酸二辛酯(DOP),邻苯二甲酸二(2-乙基己基)酯(DEHP)]同时进行定量分析的方法。最佳色谱条件:色谱柱:Agilent-C18(250mm×4.6mm,5μm);流动相:甲醇:水(V/V)=80:20(0—10min)—90:10(10—35min);流速:1.0mL/min;紫外检测器,检测波长:280nm。该方法加标回收率在99.00%—100.43%之间,相对标准偏差小于5%。

The method for the simultaneous quantitative analysis of five phthalate acid esters（PAEs） in environmental water,including dimethyl phthalate（DMP）,diethyl phthalate（DEP）,di-n-butyl phthalate（DBP）,dioctyl phthalate（DOP）,di（2-ethylhexyl） phthalate（DEHP）,was established by high performance liquid chromatograph（HPLC）.The optimal chromatograph conditions were as follows： Agilent-C18 column（250mm×4.6mm,5μm）with methyl-water[V/V;80∶20（0—10min）—90∶10（10—35min）] as mobile phase at a flow rate of 1.0mL/min with the UV detection,and the wavelength was set at 280nm.The additional standard recoveries of the method were in the range of 99.00%—100.43%,and the relative standard deviations were less than 5%.