分散液相微萃取-高效液相色谱法测定蜂蜜中的磺胺类抗生素

Determination of Sulfonamides in honey by DLLME-HPLC

应用分散液相微萃取(DLLME)技术,建立了蜂蜜中磺胺类抗生素的高效液相色谱分析方法。考察了萃取剂、分散剂、萃取剂体积、分散剂体积、时间、盐度和pH等因素对分散液相微萃取的影响,并确定了最佳萃取条件为:40μL 1,1,2,2-四氯乙烷(萃取剂)和1mL乙腈(分散剂),混匀后,加入水样,离心2min,吸取5μL沉积相,进行HPLC分析。应用本方法对3批蜂蜜样品进行了测定,取得了良好的效果。

The method for analysis of sulfonamides antibiotic in honey by dispersive liquid-liquid microextraction（DLLME） combined with high performance liquid chromatography was developed.The effects of parameters such as the extraction solvent,dispersing agent,volume of extraction solvent and dispersing solvent,extraction time,ionic strength and solution pH on dispersion of liquid phase microextraction were investigated,and the optimal extraction conditions were as follows：1mL of acetonitrile（as dispersive solvent） and 40μL 1,1,2,2-tetrachloroethane（as extraction solution） were rapidly mixed,then centrifuged for 2min after adding water solution of honey samples,and 5μL sedimentary facies was injected to analyze by HPLC.The method is applied to determination of 3 batches honey samples with good effects.