头颈部鳞癌及癌旁组织端粒酶活性检测

Telomerase activity in human head and neck squamous cell carcinoma and adjacent tissues

目的 :研究原发头颈部鳞癌及相应癌旁组织中端粒酶活性表达 ,探讨其作为头颈部鳞癌分子生物学标志物的可能性。方法 :采用 TRAP- PCR- EL ISA,对 32例原发头颈部鳞癌及 15例癌旁组织进行端粒酶活性检测。结果 :32例原发头颈部鳞癌中 ,2 7例端粒酶活化 ,阳性率为 84.4% ;15例癌旁组织中 5例端粒酶活化 ,阳性率为33.3%。有淋巴结累及者端粒酶阳性率 (86 .7% )高于无淋巴结累及者 (82 .4% ) ,低分化鳞癌中端粒酶阳性率(90 % )高于中高分化磷癌端粒酶阳性率 (81.8% ) ,但差异均无显著性。端粒酶活性与肿瘤原发部位、临床分期无相关性。结论 :端粒酶活化与头颈部鳞癌的发生发展有密切关系 ,其可望成为头颈部鳞癌的分子生物学标志物。

Objective:To investigate the telomerase activity in human head and neck squamous cell carcinomas and their adjacent tissues and to explore its possibility of being tumor biological marker.Method:Thirty two patient samples of primary head and neck squamous cell carcinoma and 15 adjacent tissues were assayed using TRA PCR ELISA for detection of telomerase activity.Result:Twenty seven of 32 ( 84.4 %) cancer samples and 5 of 15( 33.3 %) adjacent samples were telomerase positive (P< 0.05 ,Chi Square Test).The frequency of telomerase activation was lower in cancer without lymphnode involvement ( 82.4 %) than that with lymph node involvement( 86.7 %), but the difference has no statistical significance.Detection rate of telomerase activity in poor grading cancer (90%) is higher than that in moderate and high differentiation cancer ( 81.8 %),although no statistical significance.No obvious correlation was found between the telomerase activity and the other clinical parameters such as primary site and staging.Conclusion:The finding of telomerase activity in 84.4 % of primary head and neck squamous cell carcinoma samples is consistent with the concept of telomerase playing a key role in tumorigenesis.Further study is needed to determine the usefulness of this enzyme as a new molecular marker.