喉乳头状瘤HPV_(16/18)感染与p53蛋白表达的相关性

Study on the association between HPV_(16/18) and expression of p53 protein in laryngeal papillomas

目的 :了解喉乳头状瘤组织内 HPV1 6 / 1 8的感染与抑癌基因 p5 3变异的关系 ,以及 HPV感染在喉乳头状瘤发病中的作用。方法 :采用 PCR和免疫组化技术 ,检测 35例喉乳头状瘤组织中 HPV1 6 / 1 8DNA及 p5 3蛋白的表达。结果 :2 4例组织中检出 HPV1 6 / 1 8DNA(6 8.6 % ) ;19例 p5 3蛋白呈过度表达 (5 4.3% ) ;在 12例中同时检出HPV1 6 / 1 8DNA和 p5 3蛋白过度表达 (34 .3% )。结论 :提示 HPV1 6 / 1 8感染和 p5 3变异与喉乳头状瘤的发生明确相关 ,其内在分子机理及临床意义有待进一步阐明。

Objective:To understand the relationship between the expression of p53 protein and HPV 16/18 infection in laryngeal papillomas.Method:using PCR and immunohistochemistry techiques examined the paraffin embedded tissues of 35 cases of laryngeal papillomas.Result:showed that HPV 16/18 DNA were found in 24 cases of laryngeal papillomas( 68.8 %);overexpression of p53 protein were detected in 19 cases( 54.3 %);both of HPV 16/18 DNA and overexpression of p53 protein were demonstrated in 12 cases of laryngeal papillomas( 34.3 %).Conclusion:This study suggests that HPV 16/18 infection and p53 gene mutation are associated with pathogenesis of laryngeal papillomas. The relation between HPV infection and p53 mutation in tissues of laryngeal papillomas remains to be clarified.