青草沙水库中一株铜绿微囊藻抑藻细菌的作用研究

Study on inhibitory effect of an anti-Microcystis aeruginosa bacterium strain from Qingcaosha Reservoir on Microcystis aeruginosa

【目的】为了研究青草沙水库中土著微生物对藻类生长的抑制作用,从水库水体中筛选出对藻类有抑制作用的细菌并研究其对铜绿微囊藻的抑制效果。【方法】通过对水库水体中的细菌进行划线分离和筛选,挑选出一株对铜绿微囊藻生长有较好抑制作用的菌株CL。考察其对铜绿微囊藻的抑制效果及不同培养时间和菌液浓度对抑藻效果的影响,并对菌株进行16S rDNA序列分析。【结果】实验菌液浓度为4.5×108CFU/mL 8.4×108CFU/mL时,细菌对铜绿微囊藻的抑制率可达45.4%。抑藻效果随培养时间先增后降,在静置培养第6天抑藻效果达到最大。该菌经过16S rDNA序列分析,属于黄单胞菌科的寡养单胞菌。【结论】从青草沙水库中筛选出了对铜绿微囊藻有抑制作用的土著细菌寡养单胞菌,对青草沙水体铜绿微囊藻的控制具有一定的潜在应用价值。

[Objective] In order to study control strategy of the Microcystis aeruginosa by na-tive microorganism from Qingcaosha Reservoir water,the anti-Microcystis aeruginosa bacte-rium was isolated from Qingcaosha Reservoir water and its inhibitory effect on Microcystis aeruginosa was investigated.[Methods] Based on isolating and screening the bacteria cultured from the reservoir water,a strain CL capable of inhibiting Microcystis aerginosa was obtained.The inhibiting performance and influences parameters,such as culture time and concentration of bacterium on inhibitory effect were studied and 16S rDNA sequence analysis was done.[Results] Results showed that the inhibitory ratio would reach 45.4% when bacterium concentra-tion ranged from 4.5×108 CFU/mL to 8.4×108 CFU/mL.Inhibitive effect first increased and then dropped with the culture time,the maximum inhibitory ratio appeared in the sixth day.16S rDNA sequence analysis revealed that the strain CL belongs to Stentrophomonas genus of Xanthomonas family.[Conclusion] A native bacteria named Stentrophomonas capable of inhibiting Microcystis aeruginosa was isolated from Qingcaosha Reservoir water,which has a certain potential use in controlling Microcystis aeruginosa of Qingcaosha Reservoir.