HPLC测定微球中盐酸左氧氟沙星的含量

Determination of Levofloxacin Hydrochloride in Microspheres by HPLC

目的建立测定壳聚糖微球中盐酸左氧氟沙星含量的高效液相色谱法。方法采用Agilent 1200型高效液相色谱仪,色谱柱为ZORBAX Eclipse XDB-C18（4.6 mm×150 mm,5μm）,柱温：30℃,流速：1.0 mL.min 1,流动相：0.04 mol.L 1磷酸溶液（三乙胺调pH值为4）-乙腈（85∶15）,检测波长：293 nm。结果盐酸左氧氟沙星浓度在1.021~51.15μg.mL 1内线性关系良好（r=0.999 6）,日内和日间精密度RSD均〈2%（n=5）,平均加样回收率为99.1%,RSD为0.72%。结论本方法简便易行、灵敏度高、重复性好,结果准确可靠,适用于壳聚糖微球中盐酸左氧氟沙星含量测定。

OBJECTIVE To establish an HPLC method for determination of levofloxacin hydrochloride in chitosan microspheres.METHODS The chromatographic analysis was performed on a ZORBAX Eclipse XDB-C18 column（4.6 mm×150 mm,5 μm） at 30 ℃.The mobile phase was a mixture of 0.04 mol·L 1 phosphoric acid（adjusted to pH 4 with triethylamine） and acetonitrile（85∶15）.Flow rate was 1.0 mL·min 1 and UV detection wavelength was 293 nm.RESULTS Good liner relationship was obtained in the range of 1.021 51.15 μg·mL 1（r=0.999 6）.Both intra-and inter-day precision RSD were less than 2%（n=5）.The average recovery rate was 99.1% with RSD of 0.72%.CONCLUSIONS The established method is sensitive,reliable and suitable for quantitative analysis of levofloxacin hydrochloride in chitosan microspheres.