P53基因突变与肺癌预后复发转移的关系

P53 gene mutation in lung cancer and their effect on patients' survival and metastasis

目的 探讨 P53基因突变与肺癌病理特征、预后、复发转移的关系。方法 聚合酶链反应 -单链构象多态性分析 (PCR- SSCP)方法检测 155例原发肺癌手术标本中 P53基因 5～ 8外显子突变。结果 小细胞肺癌 (SCL C) P53突变率 80 % ,非小细胞肺癌 (NSCL C) 53.8% (78/ 145)。 94例随访病人中 ,P53阳性组一年存活率为 6 8.6 0 % ,而 P53阴性组为 97.7% (P<0 .0 5)。P53突变组术后复发转移率 80 .6 % ,无突变组 2 8%。结论 P53突变是肺癌常见的基因异常。 SCL C突变率高于NSCL C。 P53突变与 NSCL C肿瘤大小、淋巴结转移及患者术后存活时间和复发转移关系密切 ,可作为 NSCL

Objective To explore the relationship between P53 gene mutation and clinical characteristics in lung cancer patients.Methods DNA samples were obtains from 155 patients with lung cancer who underwent pulmonary resection from 1991 to 1997 in our institution.Mutations of P53 gene occurring at exon 5～8 were detected by a polymerase chain reaction product — single strand conformation polymorphism (PCR SSCP).Results Eighty percent P53 mutation in SCLC and 53.5% in NSCLC were detected.Ninety four NSCLC patients were followed up more than one year after surgery.One year survival rate was 68.6% (35/51) in NSCLC patients with P53 mutation,and 97.7% (42/43) in patients without P53 mutation ( P <0.05).The frequency of metastasis and recurrence in NSCLC patients with P53 mutation (80.6%) was significantly higher than those without P53 mutation (28%).Conclusions P53 mutation can happen in early stage of lung cancer.The P53 gene mutation is poor an indicator for prognosis and metastasis in patients with NSCLS.