ITS序列在酵母批量分子鉴定中的适用性

Internal Transcribed Spacer(ITS) Sequence as Measure for Systemic Classification of Yeasts

内部转录间隔区(internal transcribed spacer,ITS)序列是目前最常用的酵母分子鉴定标识之一。该文利用ITS序列同源性分析法对保藏于中国高校工业微生物资源与信息中心的623株酵母分离物进行了鉴定。实验结果显示:581株酵母分离物(93.3%)可通过ITS序列直接鉴定到种一级,40株分离物(6.4%)可鉴定至属一级,仅有2株酵母分离物无法通过ITS序列获得有效鉴定。上述结果表明,ITS序列在大多数酵母种属鉴定中具有很高的敏感性和特异性,可以作为第一分子标识用于大批量酵母分离物的分子鉴定。

The internal transcribed spacer （ITS） region of nuclear DNA is one of the most used locus for yeast i- dentification with growing sequence data deposited in public-access databases. This study extensively evaluated the feasibility of ITS sequence analysis for systemic classification of yeast isolates. The ITS regions of 623 yeast strains de- posited in CICIM-CU were amplified by PCR and sequenced, the sequences were compared to reference data available at the GenBank database by using BLAST. Most of fungal isolates （93.3%） were assigned to species level successful- ly. 40 strains （6.4%） were only can be assigned to genus level. 2 strains cannot be identified based on ITS se- quences analysis. This study shown ITS sequences were sufficiently sensitive for identifying most of yeasts, it can be powerfully and ordinarily used as first-round barcoding gene to systemic identification of bulk yeast isolates.