纤维素酶水解能力的影响因素及纤维素结构变化研究

Study on the Factors Affecting Enzymatic Hydrolysis Capability and the Structural Changes of Cellulose

以纤维素和秸秆为底物,对纤维素酶在不同条件下的水解效率进行了研究,考察的影响因子包括时间、温度、pH值,同时利用扫描电子显微镜(SEM)分析了酶水解过程中两种底物的结构变化。研究结果表明,在不同pH条件下,纤维素水解效率有显著差异,pH4.0～5.0为其最适范围;温度对水解效率的影响很大,40℃时纤维素和秸秆生成葡萄糖效率最高,分别为56.32%和35.80%;两种底物的水解在24 h内基本完成,48 h水解效率达到最高,纤维素酶水解进行的前6 h中,底物的结构变化最为明显。

Enzymatic hydrolysis of cellulose and straw was investigated under different hydrolysis conditions, such as reaction time, temperature and pH value. The structural change of the substrates during the process was also analyzed by scanning electron microscope （SEM）. Results indicate that, the efficiency of enzymatic hydrolysis signifi- cantly varied with different pH values, and the optimal pH value was found to be in the range of 4.0 N 5.0. Moreo- ver, enzymatic hydrolysis efficiency was sensitive to temperature and the optimal value of temperature was 40 ~C. Un- der this condition, the production rate of glucose by straw and cellulose reached 56.32% and 35.80% , respectively. Furthermore, hydrolysis process of both substrates was almost completed within 24 hours, and reached the highest effi- ciency after 48 hour. SEM results show that the substrate structure underwent obvious change during the first 6 hours.