铜绿微囊藻混养生长能力及其对细胞增殖的影响研究

Mixotrophic Capability and Its Effect on the Growth of Microcystic aeruginosa

以葡萄糖为外加碳源,对不同氮磷营养水平下低光照条件中铜绿微囊藻的混养生长能力及其对细胞增殖的影响进行了系统研究,同时讨论了不同营养水平下葡萄糖对微囊藻生长的贡献差异。结果表明,铜绿微囊藻在低光照([500±50)lx]下可吸收利用外界葡萄糖维持自身生长;外界适当浓度葡萄糖会促进微囊藻增殖,但过高则会抑制其生长,且微囊藻对葡萄糖的耐受性与氮磷营养环境存在着交互关系;混养生长组中的细胞叶绿素a含量均低于光照自养组。研究还发现,相同浓度的葡萄糖在氮磷营养较低的环境中对微囊藻增殖的贡献程度远远大于氮磷含量较高的环境。本文从氮磷营养水平的角度考察铜绿微囊藻的混养生长能力,并揭示混养生长在不同营养水平下对细胞增殖的贡献,可为解释低氮磷水体中暴发水华的原因提供科学依据。

The growth and the utilization of organic carbon source（glucose） of Microcystic aeruginosa under different nitrogen and phosphorus were studied in this paper, and the contributions to the promotion of Microcystic aeruginosa at the same concentration of glucose were dis- cussed. The results indicated that glucose could be assimilated definitely by Microcystic aeruginosa to maintain its growth under low light intensity[（ 500±50 ）lx]. Meanwhile, the proper concentration of organic carbon would enhance the growth of the Microcystic aeruginosa while it would be inhibited at the high organic carbon source. Also, the tolerance of the Microcystic aeruginosa to the organic carbon source had some connections with the nutrient conditions, and the content of cell chlorophyll a in mixotrophic group was less than that of the control group. Moreover, it was also revealed that compared with the high nutrient condition, the organic carbon source made a greater contribution to the growth of Microcystic aeruginosa when placed in a low nutrient condition. The innovative point of the study lies in studying the mixotrophic growth capability from the level of nitrogen and phosphorus content and reveals the contribution to the cell growth under different nutrient conditions, which could give a key clue about the reason why the water blooms develops in the waterbody where N＆P was in low concenration.