鸡胰岛素样生长因子1受体基因G26336A、C111014A多态位点的检测及其与生产性状的相关性

G26336A and C111014A Polymorphism of IGF1R Gene and its Association with Growth and Reproductive Performance in Chickens

根据GenBank发表的鸡胰岛素样生长因子1受体(insulin-like growth factor 1receptor,IGF1R)基因序列针对外显子2和3设计2对引物,采用DNA池测序和PCR-SSCP技术,对鸡IGF1R基因的G26336A、C111014A位点进行多态性检测,并与鸡的生产性状进行相关性分析。结果表明,G26336A位点AA基因型个体56日龄体重显著低于BB基因型个体(P<0.05),AA和AB基因型个体在84日龄体重显著低于BB基因型个体(P<0.05)。C111014A位点CC、CD基因型个体初生重显著低于DD基因型(P<0.05),CC基因型个体在300日龄体重显著低于DD基因型(P<0.05)。研究结果初步表明,IGF1R基因可能是影响鸡生长性状的一个主效基因或是与之存在紧密遗传连锁的一个标记。

According to GenBank published sequences for the chicken IGF1R gene, 2 pairs of primers were designed for exons 2 and 3, the G26336A, Cl11014A loci polymorphism for IGF1R gene of chicken were detected by using the method of DNA sequencing and use of pool PCR-SSCP technique, and the correlation analysis of production traits of chicken were also studied. The results showed that AA genotype of G26336A sites showed a significant lower than BB genotype （P〈0.05） for 56 days of age body weigh, AA and AB genotype showed a significant lower than BB genotype （P〈0.05） for 84 days of age body weight. CC, CD genotypes of Cl11014A site for birth weight was significantly lower than the DD genotype （P〈0.05）, CC genotype for the 300 days of age body weight was significantly lower than DD genotype （P〈0.05）. The results prelimina- rily showed that the IGF1R gene is either a major gene that influences the body weight traits of chicken or a molecular marker in close linkage with such a gene.