不孕妇女种植窗期血小板活化因子及其受体的表达和意义

Expression of platelet-activating factor and its receptor at implantation window stage among infertile women

目的:探讨血小板活化因子(PAF)及其受体(PAF-R)在不孕妇女种植窗期的表达及意义。方法:在10例不孕妇女(不孕组)和10例正常非孕妇女(正常组)种植窗期及10例妊娠早期妇女(孕40+天,孕早期组),①采用ELISA法检测3组对象血清PAF的含量及血小板活化因子-乙酰水解酶(PAF-AH)的活性;②流式细胞仪检测3组对象外周血单核细胞上PAF-R的表达;③免疫组织化学技术检测种植窗期子宫内膜及早孕蜕膜组织中的PAF-R表达。结果:不孕组妇女种植窗期血清中PAF含量(7.49±0.90ng/ml)、PAF-AH活性(15.37±2.19ng/ml)、PAF-R在外周血单核细胞上(40.79±16.10%)和在子宫内膜组织中(0.27±0.05%)的表达与正常组和孕早期组比较差异有统计学意义(P均<0.01)。不孕组妇女种植窗期PAF-R在外周血单核细胞上和在子宫内膜组织中的表达呈正相关(r=0.972,P<0.01)。结论:①不孕妇女种植窗期血清PAF水平明显降低,PAF-AH活性明显增高,PAF-R在外周血单核细胞上和子宫内膜组织中的表达降低,可能是导致不孕的因素之一,提示PAF、PAF-AH、PAF-R表达水平的变化在女性不孕症发生机制中可能起一定作用。②外周血单核细胞上PAF-R的表达可间接反映子宫内膜组织中PAF-R的表达情况。

Objective： To explore the expression and clinical significance of platelet-activating factor （PAF） and its receptor （PAF-R） during the period of the implantation window among infertile women. Methods： Ten infertile women, 10 normal non-pregnant women and 10 pregnant women with a gestational age of 40＋ days were recruited. ELISA assay were conducted to analyze the level of PAF and the activity of PAF-acetyl-hydrolase （PAF-AH）. The expression of PAF-R on peripheral blood moncytes （PBMCs） was examined by cytoflowmetry, and levels of PAF-R in endometrum and decidual tissues were examined by immunohistochemistry method. Results： The concentration of PAF （7.49±0.90 ng/ml）, the activity of PAF-AH （15.37±2.19 ng/ml）, and the expression of PAF-R on PBMCs （40.79±16.10%） and in endometrium （0.27±0.05%） during the period of implantation in the group of infertile women were significantly lower than those in the other two groups （P all〈0.01）. The expression of PAF-R on PBMCs was positively correlated with that in the endometrium during the period of the implantation among infertile women （r= 0.972, P〈0.01）. Conclusion： Decreased concentration of plasma PAF, increased activity of PAF-AH and decreaed expressions of PAF-R on PBMCs and in endometrium could be one of the mechanisms of infertility. The expression of PAF-R on PBMCs can indirectly reflect the expression of PAF-R in the endometrium.