C-fos诱导生长因子在胰腺再生中的重要作用

The important role of c-fos-induced growth factor in the pancreas regeneration

目的探讨c-fos诱导生长因子(Figf)对小鼠胰腺再生的影响。方法建立小鼠胰腺损伤模型,于术前、术后12h、24h检测血糖,48h后收集剩余胰腺组织。经全基因组芯片分析、Q-PCR验证Figf发生高表达。构建Figf过表达质粒,转染胰岛内皮细胞(MS 1),36h后ELISA检测上清培养液胰岛素分泌量的变化,Q-PCR检测转染后MS 1中Pdx1、Insulin1mRNA表达水平。结果小鼠胰腺再生过程Figf mRNA表达上调。与未转染组、转染PcDNA 3.1组比较,转染Figf过表达质粒组胰岛素分泌量明显升高[未转染组(116.89±6.09)(P<0.01);转染PcDNA 3.1组(114.24±4.60)(P<0.01)],转染Figf过表达质粒组Pdx1、Insulin1mRNA表达水平均明显升高(P<0.01)。结论 MS 1中过表达Figf可使Pdx1、Insulin1mRNA表达水平升高,增加胰岛素的分泌。Figf可能在胰岛β细胞再生中发挥着重要作用,是小鼠胰腺再生的关键基因。

Objective To explore the role of c-los induced growth factor （Figf） in the pancreas regeneration. Methods Mouse model with pancreatic regeneration were established by pancreatectomy. Pancreatic function loss and regeneration was monitored by time-coursed blood glucose testing after pancreatectomy. The levels of blood glucose were measured before and 12 h and 24 h after operation. Regenerated pancreas tissues were collected 48 h after pancreatectomy for RNA isolation. Gene expression was performed using mouse whole genome chips. Differently expressed genes were verified by Q-PCR. The target gene, Figf, was selected and cloned in expression plasmid. MS 1 cells cultured in vitro were transfected with the Figf constructs, and insulin secretion by the transfected cells was detected by ELISA 36 h after cell transfection. The mRNA levels of Pdxl and insulinl genes in transfected cells were tested by Q-PCR. Results Figf expression was significantly increased in regenerating pancreas. As compared with the untransfected and vector- transfected groups, the insulin secretion in Figf transfected cells increased significantly [（ 116.89±6.09） pg/ml in the untransfected group （P〈0. 01）; and （114. 24± 4. 60） pg/ml in the vector-transfected group （P〈 0.01）]. As compared with the untransfected and vector-transfected groups, the Figf over expression group expressed significantly higher mRNA levels of Pdxl and insulinl genes （P〈 0. 01 ）. Conclusion Over expression of Figf gene in MS 1 cells can elevate the expression of Pdxl and insulin1 in mRNA levels, and increase the insulin secretion. Figf may be one of the key genes involoving in pancreas regeneration and plays an important role in β-cell regeneration.