镜鲤碱性磷酸酶和酸性磷酸酶QTL定位分析

Quantitative trait loci analysis for activity of acid phosphatase and alkaline phosphatase in mirror carp

磷酸酶普遍存在于动物的各组织中,对磷酸单酯键具有水解活性,是机体生长代谢、保持内环境稳定和维持机体健康所必需的酶。以镜鲤(Cyprinus carpio L.)全同胞家系190个个体为材料,用992对微卫星(SSR)标记进行基因组扫描,采用区间作图法,对肠组织的碱性磷酸酶和酸性磷酸酶进行了QTL定位分析。QTL检测显示:5个QTL区间与酸性磷酸酶活性相关,其中3个QTL为99%染色体水平显著性,分别位于LG2(CA2049-CA2371)、LG25(HLJ2941-HLJ3946)和LG28(CA2263-HLJ2873),另外两个QTL区间为95%染色体水平显著性,位于LG14(HLJ3275-CA174)和LG14(CA1276-CA2208),解释表形变异率范围为8.1%～38.9%;2个与碱性磷酸酶相关的QTL区间均为95%染色体水平显著性,分别位于LG8和LG13,可解释变异率分别为7.3%和7.0%。

A phosphatase is an enzyme that removes a phosphate group from its substrate by hydrolysing phosphoric acid monoesters, which is widely distributed in most organisms and expressed in lots of tissues. This phosphatase is necessary enzyme for growth, maintaining metabolic homeostasis and keeping the body healthy. In this research, a whole genome scan was conducted on 190 progenies from a full-sib family using 992 microsatellite markers and interval mapping strategy for identifying QTL of activity of acid phosphatase and alkaline phosphatase traits in intestinal tissue. The results indicated that ： five QTL affected the activity of acid phosphatase, of which three QTL were at the 99% chromosome-wide level located on the LG2 （CA2049- CA2371 ）,LG25 （HLJ2941-HLJ3946） and LG28 （CA2263-HLJ2873） and the remaining two QTL were at 95% chromosome-wide level explaining 8. 1% - 38.9% of the total variation, and two QTL affecting the activity of alkaline phosphatase were identified at 95% chromosome-wide level located at LG8 and LG13 explaining 7.3% and 7% of the total variation, respectively.