黑果枸杞耐盐机理的相关研究

Studies on the Physiological Mechanism Underlying Salt Tolerance of Lycium ruthenicum Murr

以黑果枸杞幼苗为材料,分别对其进行不同浓度NaCl(0、200、400mM)处理,以探讨盐胁迫对黑果枸杞幼苗生长及生理特性的影响。结果表明:不同浓度NaCl处理下,根的质膜透性随着处理时间的延长明显增大;400mM NaCl处理14d后,黑果枸杞幼苗根、茎、叶中H2O2和MDA的含量与对照相比均无明显差别;不同浓度NaCl处理至第7天时,根中的可溶性糖含量明显下降,而处理至第14天时,根中的可溶性蛋白质含量明显降低。不同浓度NaCl处理下,叶片和茎中的POD活性在处理后第7天与第14天均明显降低,而茎中的APX在处理后第14天亦明显降低。从各生理指标的测定结果来看,黑果枸杞叶片和茎具有较强的耐盐性,而其根部则对盐胁迫的耐受能力则相对较弱。

Lycium ruthenicum seedlings were chosen as test material,effect of different concentration of NaCl（0,200,400 mM）on seedling growth and physiological property of Lycium ruthenicum were studied.The results showed that the values of relative electrolyte leakage in roots increased significantly during the period of NaCl treatment;After NaCl treatment for 14 days,the contents of H2O2 and MDA did not change much as compared with the control;The content of soluble sugars decreased significantly in roots after 7 days of NaCl treatment,while the content of soluble protein declined after 14 days of NaCl exposure;Under different concentrations of NaCl treatment,POD activity in both leaves and stems declined dramatically after 7 and 14 days of treatment.According to the results obtained,the leaves and the stems posses relatively higher salt tolerance,while the root was relatively sensitive to salt stress.