火绒草中总黄酮的纯化工艺优选

Optimization of Purification Technology for Total Flavonoids from Leontopodium leontopodioid

目的:优选火绒草中总黄酮的大孔吸附树脂富集纯化工艺。方法:采用静态吸附/解吸与动态吸附/解吸相结合的方法,用紫外-可见分光光度法测定火绒草中总黄酮含量,筛选最佳大孔吸附树脂,并对其纯化工艺进行优选。结果:确定采用HPD100型大孔吸附树脂对火绒草中总黄酮进行富集纯化,其最佳工艺条件为以2 BV火绒草供试品溶液(生药质量浓度0.1 g·mL-1)为最大上样量,吸附速率为1 BV·h-1,吸附2 h,洗脱流速4 BV·h-1,5 BV水洗脱,4 BV 30%乙醇洗脱,3 BV 50%乙醇洗脱,收集洗脱液,减压浓缩,即得。按上述最佳纯化工艺总黄酮洗脱率>90%。结论:该优选工艺稳定可行,适合工业化生产。

Objective： To optimize enrichment and purification technology of total flavonoids from Leontopodium leontopodioid by macroporous resin. Method： Optimum macroporous resin and its purification technology was optimized by static adsorption/desorption and dynamic adsorption/desorption. The content of total fiavonoids was determined by ultraviolet and visible spectrophotometry method. Result： HPD100 macroporous resin showed good concentration and purification capabilities to total flavonoids from L. leontopodioid. Optimum technology conditions were as follows ： 2 BV sample liquid （the concentration of raw drug was0. 1 g- mL^-1 ） of L. leontopodioid was passed through HPD-100 resin in 2 h with adsorption rate of 1 BV-h-1, eluted by 5 BV water, 4 BV 30% ethanol, 3 BV 50% ethanol, respectively, elution flow rate was 4 BV·h^-1. Eluate was collected to concentrating under vacuum. Under this optimized technology, the elution rate of total flavonoids was more than 90%. Conclusion： This optimized technology was stable, feasible, it was suitable for industrial production.