民族药金耳环总多糖的提取工艺优选

Optimization of Extraction Technology for Total Polysaccharides form Asarum insigne

目的:优选金耳环中总多糖的提取工艺。方法:选取固液比、提取温度、提取时间、醇沉浓度为考察因素,以金耳环总多糖得率为评价指标,采用正交试验对其提取工艺进行优选。结果:金耳环总多糖最优提取工艺参数为液固比6∶1,80℃提取2次,每次90 min,90%乙醇沉淀。此条件下,总多糖平均得率1.82%,RSD 1.93%。结论:该优选方法简单快速,稳定可行。

Objective： To optimize extraction technology of total polysaccharides form Asarum insigne. Method： With ratio of solid-liquid, extraction temperature, extraction time and the concentration of ethanol precipitation as factors, orthogonal test was used for optimization of extraction process with yield of total polysaccharides from A. insigne as index. Result： Optimum extraction technology parameters of total polysaccharides form A. insigne were： solid-liquid ratio 6： 1, extracted 2 times at 80 ℃ , per time 90 min, the concentration of ethanol precipitation 90%. Under these conditions, the average yield of total polysaceharides was 1.82%, RSD 1.93%. Conclusion： This optimized technology was simple, rapid, had good repeatability and stability.