不同配伍配比对麻黄-桂枝药对有效成分含量的影响

Content of Active Compounds in Water Extracts of Ephedra with Cinnamomi by Compatibility of Different Ratio

目的:研究麻黄-桂枝不同比例配伍前后水煎液中有效成分含量的变化,探讨两药的配伍规律。方法:采用GC-MS测定水煎液中盐酸去甲基伪麻黄碱(NMP)、盐酸去甲基麻黄碱(NME)、盐酸麻黄碱(E)、盐酸伪麻黄碱(PE)、盐酸甲基麻黄碱(ME)的煎出量;采用HPLC测定水煎液中香豆素、桂皮醇和桂皮醛的煎出量。结果:与单煎液相比,麻黄与桂枝配伍后,随着配伍药材比例增大,水煎液中以上各有效成分含量均有不同程度的降低,5种麻黄生物碱含量下降幅度在15%～42%,香豆素、桂皮醇、桂皮醛的含量下降幅度在16%～57%。结论:麻黄-桂枝不同比例配伍后,对彼此有效成分的溶出有一定的抑制作用。

Objective： Study on the content of active compounds in water extracts of Ephedra-Cinnamomi by compatibility of different ratio and to explain the mechanism of this compatibility. Method： The content of norpseudoephedrine （NMP） , norephedrine （NME） , ephedrine （E） , pseudoephedrine （PE） and methylephedrine （ME）, which are the major active compounds of Herba Ephedra, were analyzed by gas chromatography-mass spectrometry （GC-MS）. The content of coumarin, cinnamic alcohol and cinnamaldehyde, which are the major active compounds of Cortex Cinnamomi in the extracts were analyzed by high performance liquid chromatography （HPLC）. Result： The content of NMP, NME, E, PE, ME, coumarin, cinnamic alcohol and cinnamaldehyde in water extracts of pairmedicine Ephedra-Cinnamomi was lower than that of single decoction. Conclusion： Combined Ephedra with Cinnamomi, the dissolution of chemical compounds was inhibited with each other in water extracts.